Fenofibrate Inhibited the Differentiation of T Helper 17 Cells In Vitro
Figure 1
Fenofibrate inhibited the differentiation of Th17 cells in vitro. (a) Total T cells were isolated from mouse spleens and induced differentiation to IL-17+ cells with 10 ng/mL TGF-β, 40 ng/mL IL-6, and fenofibrate final concentrations indicated. The percentage of IL-17+ cells was analyzed with flow cytometry 4 days later. Total T cells were treated as above, the mRNA level of RORγt was analyzed with real-time PCR (b), and the percentages of IL-17+ cells in CD4+ and CD8+ T cell subgroups were analyzed separately according to different staining patterns with flow cytometry (c). (d) CD4+ T cells were isolated from mouse spleens and induced to differentiate into Th17 cells with 10 ng/mL TGF-β, 40 ng/mL IL-6, and fenofibrate final concentrations indicated. The percentage of Th17 cells was analyzed with flow cytometry 4 days later. In (a and d), numbers at the corner indicate the final concentration of fenofibrate, and the percentages of IL-17+ cells were shown adjacent to the outlined areas, , * versus 0 μM group. In (b), , * versus 0 μM group. In (c), data represent one of three independent experiments.
