Fenofibrate Inhibited the Differentiation of T Helper 17 Cells In Vitro

Figure 3

WY14643, GW7647, and bezafibrate did not inhibit the differentiation of Th17 cells. Total T cells and CD4⁺ T cells were isolated from mouse spleens and induced differentiation to Th17 cells with 10 ng/mL TGF-β, 40 ng/mL IL-6, 5 μg/mL IFN-γ neutralizing antibody, 5 μg/mL IL-4 neutralizing antibody, and 20 μM WY14643 (a), 1 μM GW7647 (b), 50 μM bezafibrate (c) or solute control. 4 days later, the percentage of IL-17⁺ T cells was analyzed with flow cytometry. Numbers adjacent to the outlined areas indicate the percentage of IL-17⁺ cells. Data shown here represent one of three independent experiments.

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(b)

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