AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells

Figure 4

Induction of differentiation. Differentiation of CaCo-2 cells treated with 5 μM clofibrate, 0.1 μM AS601245, and the association of these two compounds (Clofi + AS601245). Cell differentiation was evaluated at 24 and 48 hours after the start of treatments, by measuring dome formation. 2 mM butyrate was used as a positive control. Results, expressed as number of domes per cm², are the mean ± SD of three separate experiments from three different preparations for each condition. Variance analysis: *𝑃<0.05, **𝑃<0.01 versus control; ^$$𝑃<0.01, versus clofibrate-treated cells; ^#𝑃<0.05 versus AS601245-treated cells. The picture represents a microscopic image of domes.