AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells
Figure 7
Analysis of JAK/STAT signalling. Western Blot analysis of STAT3 (a), P-STAT3 (Ser727) (b), and P-STAT3 (Tyr705) (c) proteins levels analyzed in CaCo-2 cells treated with clofibrate (Clofi) alone, AS601245 alone at the indicated concentrations, and combined treatment with 5 μM clofibrate and 0,1 μM AS601245 (Clofi + AS) and collected at 24 hours after the treatment. Graphics represent the relative quantification of protein products performed by densitometric scanning. Data were normalized by using the β-actin signal and expressed as arbitrary densitometric units. (d) Graphics represent the normalization of the protein level of P-STAT3 (Tyr705) by using STAT3 signal. Results are the mean ± SD of three separate experiments from three different preparations for each condition. Variance analysis: *, ** versus control.
