PPAR𝜶-Independent Arterial Smooth Muscle Relaxant Effects of PPAR𝜶 Agonists

Figure 3

Role of endothelium and K⁺ channels in PPARα agonist-mediated relaxations. The effects of nitric oxide synthase, cyclooxygenase, and endothelial denudation on the relaxant response of GW7647 (a) and WY14643 (c) in WT mice aortic rings (𝑛=3–5). L-NAME or indomethacin or endothelial denudation had no effect on GW7647- and WY14643-mediated aortic relaxations. Responses to GW7647 (b) and WY14643 (d) after pretreatment of aortic rings with elevated external KCI (80 mM), 10⁻⁷ M Iberiotoxin (IbTX), 3 × 10⁻³ M 4-aminopyridine (4-AP), 10⁻⁵ M glibenclamide (𝑛=3–6). Elevated KCI as well as glibenclamide resulted in attenuation of both GW7647- and WY14643-mediated relaxations. The other K⁺ channel inhibitors did not, however, alter the relaxations to PPARα agonists. Data are means ± SEM. *Indicates P ≤ 0.05.

(a)

(b)

(c)

(d)