Transcriptional Activity of PGC-1𝜶 and NT-PGC-1𝜶 Is Differentially Regulated by Twist-1 in Brown Fat Metabolism

Figure 4

Differential effect of Twist-1 on PGC-1α and NT-PGC-1α target gene expression in brown adipocytes. (a), (b), (c) Quantitative real time PCR analysis of gene expression in differentiated PGC-1α -null brown adipocytes expressing empty vector (pBABE), PGC-1α, and NT-PGC-1α with empty vector (white bars) or Twist-1 (black bars). Relative abundance of mRNA levels was normalized to that of cyclophilin mRNA. Data represent mean ± SEM of at least four independent experiments. *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001; ****𝑃<0.0001; ns: not significant.

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