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PPAR Research
Volume 2012, Article ID 320454, 7 pages
Research Article

Transcriptional Activity of PGC-1 and NT-PGC-1 Is Differentially Regulated by Twist-1 in Brown Fat Metabolism

Laboratory of Nutrient Sensing and Adipocyte Signaling, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA 70808, USA

Received 8 August 2012; Revised 5 September 2012; Accepted 7 September 2012

Academic Editor: Shihori Tanabe

Copyright © 2012 Hee-Jin Jun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1. Differential effect of Twist-1 on PGC-1α- and NT-PGC-1α-dependent induction of UCP1.

Quantitative real time PCR analysis of gene expression in differentiated PGC-1α-null brown adipocytes expressing empty vector (pBABE), PGC-1α, and NT-PGC-1α in the absence (white bars) or presence of Twist-1 (black bars). Differentiated brown adipocytes were collected without treatment with dibutyryl cAMP. Relative abundance of mRNA levels was normalized to that of cyclophilin mRNA. Data represent mean ± SEM of at least four independent experiments. ****,p < 0.0001; ns, not significant.

  1. Supplementary Figure