Rhein Reduces Fat Weight in db/db Mouse and Prevents Diet-Induced Obesity in C57Bl/6 Mouse through the Inhibition of PPARγ Signaling

Figure 2

Rhein blocks body weight gain in high-fat diet-fed C57BL/6 mice. (a) Body weight. (b) Food intake amount. (c) The mass of white adipose tissue (WAT) and brown adipose tissue (BAT). (d) The adipocyte size of BAT and WAT in μm². (e) TG and TC content in feces. (f) Body temperature at different times after cold exposure (4°C). (g) The mRNA expression level of the thermogenic genes in BAT. β-actin was used as an internal control for modifying the mRNA level. Mice were fed a high-fat diet for 8 weeks and rhein was powdered and mixed in the diet at 0.1% (wt/wt). HF: high-fat diet. RH: rhein. The food intake amount was recorded every 2 days through the treatment. Values are mean ± SE for 7 mice per group. *𝑃<0.05, **𝑃<0.01 versus HF group.

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