Rhein Reduces Fat Weight in db/db Mouse and Prevents Diet-Induced Obesity in C57Bl/6 Mouse through the Inhibition of PPARγ Signaling
Figure 4
Rhein regulates the transcription activity of PPARγ. ((a)–(c)) The transcription activity of PPARα, γ, β/δ. GAL4-DBD-LBD expression plasmids and a GAL4-responsive luciferase reporter were cotransfected into HEK293T cells for 24 h and treated with the PPARγ, PPARα, and PPARβ/δ agonist rosiglitazone, WY14643, GW0742 (10 μM), and rhein (10, 25, and 50 μM) for another 24 h. Rellina-luc was used as the transfection efficiency control, and the relative luciferase activities were measured by comparison to renilla luciferase activities. The results represent at least three independent experiments, and data are presented as means ± SE. (d) The relative expression levels of PPARγ target genes in WAT. (e) The relative expression levels of PPARγ target genes in the liver tissue. β-actin was used as an internal control for modifying the mRNA level. HF: high-fat diet. RH: rhein. Values are mean ± SE for 7 mice per group. * versus HF group.
