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PPAR Research
Volume 2012 (2012), Article ID 471524, 5 pages
http://dx.doi.org/10.1155/2012/471524
Research Article

Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer

1Department of Gynecology, Medical University of Bialystok, 15-222 Bialystok, Poland
2Department of Physiology, Medical University of Bialystok, 15-222 Bialystok, Poland
3Department of Gynecological Endocrinology, Medical University of Bialystok, 15-276 Bialystok, Poland

Received 11 July 2011; Revised 7 November 2011; Accepted 21 November 2011

Academic Editor: Ruth Roberts

Copyright © 2012 Paweł Knapp et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Peroxisome proliferator-activated receptors (PPARs) belong to a family of nuclear hormone receptors acting as transcriptional factors, recently involved also in carcinogenesis. Present study was undertaken to evaluate the presence and subcellular localization of different PPAR isoforms (α, β, γ) in healthy endometrial tissue ( ) and endometrial carcinoma (FIGO I, endometrioides type, G1, ). We sought to analyze PPARs mRNA content as well as protein immunohistochemical expression that was further quantified by Western Blot technique. For both PPARα and PPARβ, protein expression was significantly higher in endometrial cancers compared to normal endometrial mucosa. In opposite, PPARγ protein expression was lower in endometrial cancer cells. In each case, immunohistochemical reaction was confined to the perinuclear and/or nuclear region. At the transcriptional level, the content of mRNA of all PPAR subunits did not follow the protein pattern of changes. These results provide evidence for altered PPAR's protein expression and disregulation of posttranslational processes in endometrial cancers.