Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease
Figure 1
Cellular expression and distribution of Scd1 in rodent polycystic kidneys. (a) Relative gene expression levels for Scd1. mRNA expression levels are shown for vehicle-treted (CONT) or PIO-treated SD and PCK kidneys as compared to vehicle-treated (CONT) SD kidneys (set to 1.0) (* SD (CONT) versus PCK (CONT), # PCK (CONT) versus PCK (PIO)). Expression levels were normalized to GAPDH. (b) Renal Scd1 distribution in vehicle-treated (CONT) or PIO-treated SD and PCK rats. Representative kidney sections from vehicle-treated (CONT) or PIO-treated SD and PCK rats were stained with an antibody to Scd1. Mouse IgG2b, κ isotype control antibody, did not show any reaction in the kidney. (c) Ratio of Scd1-positive cysts or noncystic tubules in kidney sections. Positive-stained cysts or non-cystic tubules were counted in five random fields of kidney sections obtained from five rats in each group by a naive observer using a 20x objective. (** PCK (CONT) versus PCK (PIO) in noncystic tubules in the kidney section).
