PPAR Research / 2012 / Article / Tab 2

Research Article

PPARα Activation Protects against Anti-Thy1 Nephritis by Suppressing Glomerular NF-κB Signaling

Table 2

Primer sequences for quantitative real-time PCR assay.

Gene namePrimersGenBank access no.

PPARαForward: 5′-GACAAGGCTCAGGATACCACTATG-3′
Reverse: 5′-TTGCAGCTTCGATCACACTTGTC-3′
NM_013196
ACOXForward: 5′-GGGCCTGACAGAAGCCTACAAG-3′
Reverse: 5′-AAGGTCGACAGAGGTTAGGTTCCA-3′
NM_017340
IκBαForward: 5′-TGACCATGGAAGTGATTGGTCAG-3′
Reverse: 5′-GATCACAGCCAAGTGGAGTGGA-3′
NM_001105720
COX2Forward: 5′-GCGACTGTTCCAAACCAGCA-3′
Reverse: 5′-TGGGTCGAACTTGAGTTTGAAGTG-3′
NM_017232
ICAM1Forward: 5′-ACAAGTGCCGTGCCTTTAGCTC-3′
Reverse: 5′-GATCACGAAGCCCGCAATG-3′
NM_012967
TNFαForward: 5′-AACTCGAGTGACAAGCCCGTAG-3′
Reverse: 5′-GTACCACCAGTTGGTTGTCTTTGA-3′
NM_012675

PPARα, peroxisome proliferator-activated receptor α; ACOX, acyl-CoA oxidase; IκBα, inhibitory factor κBα; COX2, cyclooxygenase-2; ICAM1, intercellular adhesion molecule-1; TNFα, tumor necrosis factor-α.

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