Figure 3: ROSI affects the expression of genes coding for proteins involved in lipid synthesis in GMECs through PPARγ signaling. Dairy goat mammary epithelial cells were treated with ROSI and harvested at 0, 12, and 24 h. (a) Genes related to lipid droplet formation (PLIN2 and PLIN3) and hydrolysis of triacylglycerols (PNPLA2). (b) Genes related to fatty acid synthesis (FASN and ACACA) and desaturation (SCD). (c) Genes related to cellular fatty acid uptake (FABP3, LPL). (d) Genes related to regulation of transcription (SREBF1 and NR1H3). The data are mean ± SD of three independent experiments. versus the control group (0 h). versus the control group (0 h).