Synergism between cAMP and PPAR Signalling in the Initiation of UCP1 Gene Expression in HIB1B Brown Adipocytes
Figure 2
Luciferase reporter assay showing the effect of forskolin and rosiglitazone on (a) 3.1 kbp UCP1-Luc, (b) 260 bp UCP1-Luc, (c) 2.6 kbp PGC1-Luc, (d) 264 bp PGC1-Luc, (e) CRE-mut 2.6 kbp PGC1-Luc, (f) , and (g) , promoter activities. HIB-1B cells were grown to confluence and then transiently transfected with the above -pGL3-Luc reporter construct, as indicated. Twenty-four hours later, cells were treated with DMSO (control), rosiglitazone (10 M) for 24 hours, or forskolin (10 M) for the final 12 hours of rosiglitazone treatment, in serum-free medium, before luciferase activity was measured. Values were expressed as fold increase of ratio of firefly to renilla. Error bar means the mean sem. of three observations within a single experiment performed in triplicate. *** Significant difference , respectively, with respect to control; ### significant difference from wildtype due to the mutation of CRE.
