Effects of PPARγ Agonist Pioglitazone on Redox-Sensitive Cellular Signaling in Young Spontaneously Hypertensive Rats
Effect of pioglitazone administration on SOD1 and SOD2 protein levels and total SOD activities in the left ventricle and brainstem of young SHRs. (a) Western blot records documenting SOD1, SOD2, and GAPDH protein levels in fraction isolated from the tissues of the left ventricle of control and PIO-treated group. (b) Western blot records documenting SOD1, SOD2, and GAPDH protein levels in brainstem. (c) Quantification of SOD2 protein levels normalized to the GAPDH protein levels. Data were obtained from Western blot records and each bar represents mean ± SEM of 7 tissue samples per group. *, PIO versus control. (d) The SOD activities were analyzed using the SOD Assay kit (Fluka) in tissue samples of left ventricle and brainstem. The specific activities are expressed in units per mg of proteins and are presented as mean ± SEM, . *, PIO versus control.