Pseudoginsenoside F11, a Novel Partial PPARγ Agonist, Promotes Adiponectin Oligomerization and Secretion in 3T3-L1 Adipocytes
Figure 1
p-F11 promotes preadipocyte differentiation by activating PPARγ. 3T3-L1 preadipocytes were induced to differentiate with 0, 20, 40 μM Pseudoginsenoside F11 (p-F11) or 0.5 μM rosiglitazone (Rosi) in the absence or presence of 20 μM GW9662 for 8 days. (a) The cells were stained with Oil Red O. Quantification of the staining results was presented in (b). Results (mean ± SD, ) were expressed as a percentage of the control (p-F11 0 μM). ; . (c) The mRNA level of adiponectin and PPARγ was examined by quantitative real-time PCR. The results were expressed relative to β-actin and presented as a percentage of the control. (d) The cell lysates were subjected to 2%–15% gradient gel electrophoresis under nonreducing and non-heat-denaturing conditions to detect the three oligomeric forms of adiponectin (LMW, MMW, and HMW) (top panel). The amount of total adiponectin (Total Ad), PPARγ, or actin was detected with antibodies against the N-terminal peptide of adiponectin, PPARγ, or β-actin, respectively.
