Pseudoginsenoside F11, a Novel Partial PPARγ Agonist, Promotes Adiponectin Oligomerization and Secretion in 3T3-L1 Adipocytes
Figure 4
p-F11 inhibits PPARγ phosphorylation at Ser-273. 3T3-L1 adipocytes were pretreated with TNF-α, followed by treatment with p-F11 or Rosi for 1 hour. Phosphorylated PPARγ at Ser-273 (p-PPARγ) was detected using anti-CDK substrate antibody after immunoprecipitation with anti-PPARγ antibody. The amounts of p-PPARγ and total PPARγ were quantified and p-PPARγ/PPARγ ratio was presented as a percentage of the control (p-F11 0 μM or Rosi 0 μM).
