Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose
Expression of LCFA and LCFA-CoA binding proteins in cultured mouse primary hepatocytes. Primary hepatocytes from wild-type and L-FABP null mice were isolated from mouse livers and maintained in culture for up to four days as described in Methods. Quantitative western blotting was performed by comparison to a standard curve of known amounts of the respective recombinant L-FABP, SCP-2, or ACBP on the same blot as described in Methods. Quantitative western blots were obtained as a function of increasing time for wild-type hepatocytes in culture: (a) L-FABP; (b) SCP-2; and (c) ACBP. Time 0 = concentration in liver while time of 1–4 days indicates time in culture. For determining the effect of L-FABP gene ablation on expression of these proteins, quantitative western blots of (d) L-FABP; (e) SCP-2; and (f) ACBP were also obtained for hepatocytes from wild-type (WT) and L-FABP null (KO) hepatocytes after three days in culture. Mean ± SEM, .