PPAR Research / 2013 / Article / Fig 12

Research Article

Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose

Figure 12

Effect of TOFA and C75 on CPT1, CPT2, and ACOX1 gene expression in cultured mouse primary hepatocytes isolated from livers of wild-type (WT, L-FABP (+/+)) and null [(L-FABP ] mice. Hepatocytes isolated from wild-type [WT, L-FABP (+/+)] or gene-ablated [null, L-FABP ] mice were preincubated for 30 min with 10 μg/mL TOFA or C75 in serum-free culture medium before addition of glucose (6 or 20 mM) as described in Methods. Total RNA was isolated from hepatocytes 5 hr after glucose addition and used for quantitative real-time PCR. The fold change in CPT1A (a, b), CPT2 (c, d), ACOX1 (e, f) mRNA levels was determined relative to internal control housekeeping gene as described in Methods. Values for each genotype were expressed relative to [Alb + 6 mM glucose] within that genotype. Panels (a), (b): CPT1A mRNA fold changes in WT and L-FABP null hepatocytes; (c), (d): CPT2 mRNA fold changes in WT and L-FABP null hepatocytes; (e), (f): ACOX1 mRNA fold changes in WT and L-FABP null hepatocytes. Mean ± SEM, .

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