Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose
Displacement of L-FABP- and SCP2-bound NBD-stearic acid by TOFA (panels a and b) and C75 (panels c and d). NBD-stearic acid displacement assays were performed as shown in Methods. L-FABP or SCP-2 (25 nM in 10 mM phosphate buffer) was incubated with NBD stearate (40 nM) for 12 min at 24°C to obtain maximal fluorescence, then titrated with increasing amount of ligand. Mean ± SEM, .