Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose

Table 1

Binding constants of fatty acids, TOFA, and C75 to L-FABP and SCP-2.

LIGAND	Binding constants (𝜇M)
	L-FABP	SCP2
Fluorescent fatty acids
NBD stearate	𝐾𝑑1 = 0.018 ± 0.001; 𝐾𝑑2 = 0.055 ± 0.002	0.101 ± 0.003
Endogenous fatty acid
Stearic acid	0.087 ± 0.006^(a)	ND
Fatty acid synthesis inhibitors
TOFA	0.066 ± 0.003^(a); 0.057 ± 0.004^(c)	0.227 ± 0.020^(a)
C75	5.59 ± 0.31^(b)	not bound^(a)

𝐾_𝑖 was determined by displacing protein-bound NBD-stearic acid (a) or ANS (b) as in Methods. (c), 𝐾_𝑑 was determined by L-FABP tyrosine quenching. ND: not determined. Values are the mean ± SEM, 𝑛=3.