PPAR Research / 2013 / Article / Tab 2

Research Article

Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose

Table 2

Binding parameters for L-FABP and SCP2 to CoA interacting with fatty acid and fatty acid synthesis inhibitor CoA thioesters.

Binding constants ( M)
L-FABPSCP2

Stearoyl-CoA0.46 ± 0.03(a)ND
TOFyl-CoA0.053 ± 0.004(c)0.004 ± 0.001(a)
C75-CoA25.9 ± 3.9(b)Not bound(a)

was determined by displacing protein-bound NBD-stearic acid (a) or ANS (b) or by intrinsic L-FABP tyrosine quenching (c) as in Methods. ND: not determined. Values are the mean ± SEM, .