Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose

Table 2

Binding parameters for L-FABP and SCP2 to CoA interacting with fatty acid and fatty acid synthesis inhibitor CoA thioesters.

	Binding constants (𝜇M)
	L-FABP	SCP2
Stearoyl-CoA	0.46 ± 0.03^(a)	ND
TOFyl-CoA	0.053 ± 0.004^(c)	0.004 ± 0.001^(a)
C75-CoA	25.9 ± 3.9^(b)	Not bound^(a)

𝐾_𝑖 was determined by displacing protein-bound NBD-stearic acid (a) or ANS (b) or by intrinsic L-FABP tyrosine quenching (c) as in Methods. ND: not determined. Values are the mean ± SEM, 𝑛=3.