Interactions between Human Liver Fatty Acid Binding Protein and Peroxisome Proliferator Activated Receptor Selective Drugs

Figure 2

¹HN and ¹⁵N backbone amide chemical shift perturbations on human L-FABP produced by GW7647 and L165,041 drug binding. ((a)-(b)) A plot of the normalized chemical shift perturbations upon complex formation versus residue number. Red bars indicate highly affected residues (>0.6 p.p.m perturbation). ((c)-(d)) ¹H-¹⁵N HSQC spectrum of apo-L-FABP (red) overlaid on top of the spectrum of holo-drug L-FABP (blue).The inset shows the highly affected residues (>0.6 ppm perturbation) mapped onto the crystallographic structure of human L-FABP (PDB code: 2F73).

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