PPAR Research / 2014 / Article / Fig 3

Research Article

15-Deoxy-γ12,14-prostaglandin J2 Reduces Liver Impairment in a Model of ConA-Induced Acute Hepatic Inflammation by Activation of PPARγ and Reduction in NF-κB Activity

Figure 3

15d-PGJ2 activated PPARγ, blocked IκBα degradation, and reduced the NF-κB activation in ConA-induced hepatitis. (a) The western blot and qRT-PCR analysis of PPARγ, IκBα, and NF-κB at 6 h, 12 h, and 24 h after ConA injection in mice and effects of both low (10 μg) and high (25 μg) dose 15d-PGJ2 pretreatment groups at the same time. The phosphorylation status of NF-κB and IκBα (P-NF-κB and P-IκBα) were also detected by using western blot. The results were analyzed using Quantity One ( , for NC versus ConA, for ConA versus ConA + 10 μg 15d-PGJ2, and for ConA versus ConA + 25 μg 15d-PGJ2). (b) Immunohistochemistry used to detect the expression level of PPARγ at 24 h in all four groups. The result was analyzed using Image-Pro Plus 6.0 ( , for ConA versus ConA + 10 μg 15d-PGJ2, for ConA versus ConA + 25 μg 15d-PGJ2, and black bar for 200 μM). (c) The different expression of NF-κB evaluated by immunohistochemistry. Image-Pro Plus 6.0 was used to analyze whether there exists statistical significance among different groups ( , for NC versus ConA, for ConA versus ConA + 10 μg 15d-PGJ2, for ConA versus ConA + 25 μg 15d-PGJ2, black bar for 200 μM, and small black arrow for positive cells).
215631.fig.003a
(a)
215631.fig.003b
(b)
215631.fig.003c
(c)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.