Research Article

Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells

Figure 6

(a) Effect of γ-tocotrienol, rosiglitazone, and GW9662 given alone or in combination on PGD2 synthesis and (b) treatment effect of 15d-PGJ2 in +SA cells. +SA cells were plated at 06 cells/100 mm culture dish and treated with control or treatment media for 4-day incubation period. Afterwards, whole cell lysates were assayed for PGD2 according to the manufacturer’s protocol. Vertical bars indicate the amount of PGD2 synthesized (pg/mL) ± SEM in each treatment group. To study effect of 15d-PGJ2, +SA cells were plated at a density of 04 (6 wells per group) in 24-well culture plates and exposed to treatment media for a 4-day period. Afterwards viable cell number was determined using MTT colorimetric assay. Vertical bars indicate mean cell count ± SEM in each treatment group. * as compared with vehicle-treated controls.