PPAR Research

Research Article

Effects and Potential Mechanisms of Pioglitazone on Lipid Metabolism in Obese Diabetic KKAy Mice

Table 2

Effects of pioglitazone on the expression of cholesterol metabolism related genes in the liver of KKAy mice.


	Nor	Con	Piog

SREBP-2	0.50 ± 0.02**	1.00 ± 0.02	0.81 ± 0.03*
HMGCR	1.42 ± 0.23	1.00 ± 0.02	0.75 ± 0.07*
ApoA1	4.41 ± 0.49**	1.00 ± 0.04	0.84 ± 0.11
ApoE	0.75 ± 0.05**	1.00 ± 0.02	0.78 ± 0.05*
SR-BI	0.69 ± 0.05**	1.00 ± 0.02	0.31 ± 0.08**
LDL-R	0.82 ± 0.18	1.00 ± 0.04	1.11 ± 0.09
HL	3.03 ± 0.25**	1.00 ± 0.19	0.48 ± 0.05**
ABCA1	0.14 ± 0.01**	1.00 ± 0.05	0.61 ± 0.03**
ABCG5	1.27 ± 0.10*	1.00 ± 0.06	0.58 ± 0.05**
ABCG8	1.88 ± 0.18**	1.00 ± 0.08	0.55 ± 0.05**
Cyp7a1	1.46 ± 0.19*	1.00 ± 0.07	0.47 ± 0.03**
Cyp27a1	2.44 ± 0.09**	1.00 ± 0.17	0.60 ± 0.04**
ABCB11	2.79 ± 0.15**	1.00 ± 0.06	0.65 ± 0.06**
ABCB4	0.28 ± 0.05**	1.00 ± 0.03	0.98 ± 0.05
NPC1-L1	0.78 ± 0.24	1.00 ± 0.07	0.96 ± 0.22
ACAT-2	1.59 ± 0.17*	1.00 ± 0.04	1.48 ± 0.25
LXRα	0.99 ± 0.13	1.00 ± 0.01	0.75 ± 0.10*
LXRβ	0.93 ± 0.04	1.00 ± 0.03	0.66 ± 0.04**
FXR	0.60 ± 0.09**	1.00 ± 0.05	0.89 ± 0.07

The mRNA expression was examined by using the real-time PCR. Results are expressed as fold change, after correction for β-actin levels, relative to the control mice. Nor: C57BL/6J mice; Con: vehicle-treated KKAy mice; Piog: pioglitazone-treated KKAy mice. Data are presented as mean ± S.E.M. ( = 6–8 per group). , versus Con.
SREBP-2: sterol regulatory element binding transcription factor 1; HMGCR: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; ApoA1: apolipoprotein A-I; ApoE: apolipoprotein E; SR-BI: scavenger receptor class B, member 1; LDLR: low density lipoprotein receptor; HL: hepatic lipase; ABCA1: ATP-binding cassette, subfamily A, member 1; ABCG5: ATP-binding cassette, subfamily G, member 5; ABCG8: ATP-binding cassette, subfamily G, member 8; Cyp7a1: cytochrome P450, family 7, subfamily a, polypeptide 1; Cyp27a1: cytochrome P450, family 27, subfamily a, polypeptide 1; ABCB11: ATP-binding cassette, subfamily B (MDR/TAP), member 11; ABCB4: ATP-binding cassette, subfamily B (MDR/TAP), member 4; NPC1-L1: Niemann-Pick C1 Like 1; ACAT-2: acetyl-coenzyme A acetyltransferase 2; LXRα: liver X receptor alpha; LXRβ: liver X receptor beta; FXR: farnesoid X receptor.