Figure 1: Huaier protected podocytes against ADR-induced injury in vitro. (a) Percentage of apoptotic cells. (b) Real-time RT-PCR of nephrin and podocin. (c) Western blot of nephrin and podocin. Left: representative immunoblots. Right: densitometric analysis. Podocytes were pretreated with Huaier (0.2 mg/mL) for 1 h followed by incubation with ADR (200 nM) for further 48 h (for apoptosis analysis, (a)) or 24 h (for real-time RT-PCR analysis (b); immunoblotting analysis (c)). Data are expressed as the means ± SD (). versus control. versus ADR group.