AICAR Protects against High Palmitate/High Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression

Figure 2

AICAR upregulates the mRNA levels of the three PPARs in HL-1 cardiomyocytes. Analysis of the mRNA levels of Ppara (a), Ppard (b), and Pparg (c) by real-time RT-PCR in HL-1 cells stimulated by AICAR for 24 h in the presence or absence of Compound C. Data are normalized by the Cyclophilin A mRNA levels and expressed as mean ± SD of 4 different experiments. (, versus control nonstimulated cells; versus AICAR-treated cells).

(a)

(b)

(c)