Peroxisome Proliferator-Activated Receptor γ Induces the Expression of Tissue Factor Pathway Inhibitor-1 (TFPI-1) in Human Macrophages

Figure 4

PPARγ activation induces the expression of TFPI-1 in a PPARγ-dependent manner. (a) H3K9ac ChIP-seq signals from M2 macrophages (Mac.) as well as PPARγ ChIP-seq signal from human primary adipocytes (Ad.) are shown for the TFPI-1 gene. Active regulatory regions are highlighted in gray and chromosomal localization (Hg18) and sequences of PPRE identified within these regions are provided at the bottom. (b) Differentiated macrophages were transfected with scrambled or human PPARγ siRNA and subsequently treated with GW1929 (600 nM) or DMSO (Control) during 24 h or were infected with a GFP (Ad-GFP) or a PPARγ (Ad-PPARγ) adenovirus and then treated with rosiglitazone (24 h, 100 nM) (c). Statistically significant differences between treatment and control groups are indicated (; ).

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(b)

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