IL-15 Mediates Mitochondrial Activity through a PPARδ-Dependent-PPARα-Independent Mechanism in Skeletal Muscle Cells

Figure 1

Effect of IL-15 signaling on mitochondrial activity. (a) Representative images of mitochondrial activity assessment in live cells using a fluorescent probe sequestered into active mitochondria; (b) quantifiable fluorescence corrected for myotube size; (c) western blot of PPARα and PPARδ protein expression; (d) mRNA expression of IL-2Rγ. Assessments were carried out on differentiated C2C12 myotubes following treatment with 100 ng/mL of IL-15 every other day for 6 days during the differentiation protocol. Image J was used to quantify cell fluorescence. GAPDH was used as control for protein and mRNA expression assessments. All values are displayed as mean ± SEM, = 3–6 per group, .

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