IL-15 Mediates Mitochondrial Activity through a PPARδ-Dependent-PPARα-Independent Mechanism in Skeletal Muscle Cells

Figure 5

Effects of PPARδ inhibition on IL-15 mediated alterations of mitochondrial associated factors. (a) mRNA expression of PPARδ following inhibition with GSK-3787 (GSK); (b) mRNA expression of PPARα following exposure to GSK; (c) mRNA expression of PGC1α, PGC1β, UCP2, and Nrf1 with IL-15 treatment in combination with GSK. Throughout differentiation, cells were treated every other day, for 6 days, with either vehicle control (DMSO), IL-15 (100 ng/mL), 1 μM of the PPARδ inhibitor (GSK-3787), or IL-15 + GSK-3787 (I + G). GAPDH was used as an internal control for qPCR analysis. All values are displayed as means ± SEM, = 6–9 per group, from all groups; .

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