PPAR Research

Research Article

Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells

Table 2

mRNA expression of PPARs-responsive genes in PPAR-transfected MCF-7 cells (with overexpression of PPARs) after treatment with experimental FA or specific agonist/antagonist of PPAR for 48 h.
Gene symbolFC values ± SD
EFA versus NCEFA-CLA versus NCcis9,trans11CLA versus NCtrans10,cis12CLA versus NCAgonist versus NCAntagonist versus NC
(A) mRNA expression of PPARα-dependent genes
TCF-20
WT1−1,86
ZNF621
THRB (TRβ2)
(B) mRNA expression of PPARδ-dependent genes
TCF-20
WT1
ZNF621
THRB (TRβ2)
(C) mRNA expression of PPARγ-dependent genes
TCF-20
WT1
ZNF621
THRB (TRβ2)
FC: fold change; NC: negative control. Agonist/antagonist: for PPARα, WY14643/GW-6471; for PPARδ, GW-0742/GSK0660; for PPARγ, troglitazone/T0070907. .