Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression

Table 1

Pgc-1α isoform primer pair sequences are shown for the forward (F) and reverse (R) primers used to measure isoform expression via qRT-PCR. Primer pairs have previously been reported by Miura et al. 2008, Ruas et al. 2012, and Zhang et al. 2009.

Primer target	Primer sequence (5′ to 3′)
Pgc-1α-a F	GCTTGACTGGCGTCATTCG
Pgc-1α-a R	ACAGAGTCTTGGCTGCACATGT
Pgc-1α-b F	GACATGGATGTTGGGATTGTCA
Pgc-1α-b R	ACCAACCAGAGCAGCACATTT
Pgc-1α-c F	AGTGACATGGATGTTGGGATTG
Pgc-1α-c R	GAATGCCTCCGGTTACTCACTT
Pgc-1α4 F	TCACACCAAACCCACAGAAA
Pgc-1α4 R	CTG GAA GAT ATG GCA CAT
FL-Pgc-1αF	TGCCATTGTTAAGACCGA
FL-Pgc-1αR	CCAGAGTCACCAAATGACC
total NT-Pgc-1α F	TGCCATTGTTAAGACCGA
total NT-Pgc-1α R	CCATATCTTCCAGTGACC
total Pgc-1α F	TGATGTGAATGACTTGGATACAGACA
total Pgc-1α R	GCT CAT TGT TGT ACT GGT TGG ATA TG