Caffeoylquinic Acid-Rich Extract of Aster glehni F. Schmidt Ameliorates Nonalcoholic Fatty Liver through the Regulation of PPARδ and Adiponectin in ApoE KO Mice

Figure 6

Succinate dehydrogenase activity, triglyceride, interleukin 6 (IL6), and reactive oxygen species (ROS) concentrations, and hematoxylin and eosin staining in liver of ApoE KO mice treated with 0.15% cholesterol diet and Aster glehni extract. (a) Succinate dehydrogenase (SDH) activity in liver protein extracts of ApoE KO mice was estimated with colorimetric method containing nitroblue tetrazolium reagents. (b) Triglyceride (TG) contents in liver extracts of ApoE KO mice were estimated with triglyceride colorimetric assay kit. (c, d) IL6 and ROS contents in liver extracts of ApoE KO mice were estimated with IL6 and ROS colorimetric assay kits. (e) Liver tissue slides of ApoE KO mice were fixed and stained by hematoxylin and eosin reagents. Magnification is 200 times. The results are expressed as means ± SEM. Values were statistically analyzed by unpaired -test and one way ANOVA. All experiments were repeated three times. Liver cells having representative ballooning morphology were indicated by arrow marks (). versus control, versus control, and versus control.

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