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PPAR Research
Volume 2017 (2017), Article ID 4089214, 12 pages
https://doi.org/10.1155/2017/4089214
Research Article

Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease

1Department of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China
2Human Movement Science, Hebei Institute of Physical Education, Shijiazhuang, Hebei 050041, China
3Department of Endocrinology, Hebei General Hospital, Shijiazhuang, Hebei 050051, China

Correspondence should be addressed to Zhenlong Guan; nc.ude.utbeh.liam@06nauglz

Received 29 August 2016; Revised 8 December 2016; Accepted 21 December 2016; Published 5 March 2017

Academic Editor: Maria Paola Cerù

Copyright © 2017 Yanqin Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Effect of 2,4-thiazolidinedione on the survival rate induced by with MTS assay in SH-SY5Y. The survival rate was found to be dramatically decreased in MPP+ group, 2,4-TZD intervention made the survival rate significantly increased in 0.01, 0.1, 1, and 10 μmol/L 2, 4-TZD treatment groups. The intervention effects were better in 0.01, 0.1, 1μmol/L 2, 4-TZD treatment groups than in 10 μmol/L 2, 4-TZD treatment groups. “aa” indicates P< 0.01, compared with control group; “b” indicates P< 0.05, compared with MPP+ group; “bb” indicates P < 0.01, compared with MPP+ group.

Comparison of cell apoptosis percentage of each group detected with Hoechst33342 staining. The apoptosis rate was dramatically increased in group compared to control group, intervention made the apoptosis rate reduced compared to MPP+ group. There is no significant difference in 0.01, 0.1, 1 μmol/L 2, 4-TZD treatment group. The apoptosis rate of 10 μmol/L 2, 4-TZD treatment group raised obviously. “aa”indicates P< 0.01, compared with control group; “bb” indicates P< 0.01, compared with MPP+ group; “cc” indicates P < 0.01, compared with 0.01μmol/L 2, 4-TZD treatment group; “dd” indicates P < 0.01, compared with 0.1μmol/L 2, 4-TZD treatment group; “ee” indicates P < 0.01, compared with 1μmol/L 2, 4-TZD treatment group.

Effect of 2, 4-TZD on MPP + induced apoptosis by Fluoro-Jade C(FJC) staining in SH-SY5Y. FJC is a green fluorescent anion derivatives labeling the degenerating neurons. Under inverted fluorescence microscope, took photos of all cells in the bright field and then took photos of apoptotic cells with FITC channel, finally calculated cell apoptosis rate. The apoptosis rate was dramatically increased in group compared to control group, intervention made the apoptosis rate reduced compared to MPP+ group. “aa” indicates P< 0.01, compared with control group; “bb” indicates P< 0.01, compared with MPP+ group; “c” indicates P < 0.05, compared with 0.01μmol/L 2, 4-TZD treatment group, “cc” indicates P < 0.01, compared with 0.01μmol/L 2, 4-TZD treatment group; “dd” indicates P < 0.01, compared with 0.1μmol/L 2, 4-TZD treatment group; “ee” indicates P < 0.01, compared with 1μmol/L 2, 4-TZD treatment group.

  1. Supplementary Material