Chiglitazar Preferentially Regulates Gene Expression via Configuration-Restricted Binding and Phosphorylation Inhibition of PPARγ

Figure 6

Chiglitazar differentially affects promoter binding of PPARγ-containing transcription complexes. HPA-v cells were incubated with Chi (1 μM), Ros (1 μM), Pio (1 μM), and vehicle control (0.1% (vol/vol) DMSO) in the presence or absence of TNFα (5 ng/ml) for 24 hours. ChIP assays using anti-PPARγ antibody were then performed as described in Materials and Methods. The relative abundance of promoters bound to PPARγ was normalized against the input control sample. The diagrammed results represent the average of two independent experiments. represents statistically significant differences in promoter binding induced by chiglitazar compared to the other two TZDs ().

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