Fimasartan Ameliorates Nonalcoholic Fatty Liver Disease through PPARδ Regulation in Hyperlipidemic and Hypertensive Conditions

Figure 5

Peroxisome proliferator-activated receptor delta (PPARδ), 5′ adenosine monophosphate-activated protein kinase (AMPK), and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein expressions, succinate dehydrogenase (SDH) activity, and adenosine triphosphate (ATP) concentration in liver tissues of in WKY rats and SHRs given high-fat diet and fimasartan. ((a), (b), and (c)) Protein extracts were electrophoresed in 10% polyacrylamide gel and blotted to nitrocellulose membrane. The nitrocellulose membrane was bound with primary and secondary antibodies sequentially, and then the chemiluminescent signal was exposed to X-ray film. The density of bands on X-ray film was analyzed with ImageJ program. (d) The SDH activity was assayed by enzymatic colorimetric method. (e) The concentration of ATP in liver extracts of WKY rats and SHRs was estimated with ATP ELISA assay kit. The results are expressed as means ± SEM ( or 6). Values were statistically analyzed by unpaired t-test. All experiments were repeated three times. Meaning of indications: WKY means normal control rat given normal diet containing 10% fat, S10 means SHRs given normal diet containing 10% fat, S10F means SHRs given normal diet containing 10% fat plus fimasartan, S60 means SHRs given high-fat diet containing 60% fat, and S60F means SHRs given high-fat diet containing 60% fat plus fimasartan.

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