Fimasartan Ameliorates Nonalcoholic Fatty Liver Disease through PPARδ Regulation in Hyperlipidemic and Hypertensive Conditions

Figure 7

11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSDH1) protein expression, hematoxylin and eosin staining, and the concentration of triglyceride in the liver tissue samples of WKY rats and SHRs given high-fat diet and fimasartan. (a) Liver tissue sections obtained from WKY rats and SHRs were fixed and stained by immunohistochemistry method for 11β-HSDH1. Magnification is 100x. (b) The densities for images were analyzed with the ImageJ program. (c) Liver tissue sections obtained from WKY rats and SHRs were fixed and stained with hematoxylin and eosin reagents. Magnification is 200x. (d) Triglyceride contents in liver extracts obtained from WKY rats and SHRs were determined with triglyceride colorimetric assay kit. The results are expressed as means ± SEM ( or 6). Values were statistically analyzed by unpaired t-test. All experiments were repeated three times. Meaning of indications: WKY means normal control rat given normal diet containing 10% fat, S10 means SHRs given normal diet containing 10% fat, S10F means SHRs given normal diet containing 10% fat plus fimasartan, S60 means SHRs given high-fat diet containing 60% fat, and S60F means SHRs given high-fat diet containing 60% fat plus fimasartan.

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