Expression of GPR43 in Brown Adipogenesis Is Enhanced by Rosiglitazone and Controlled by PPARγ/RXR Heterodimerization

Figure 2

Rosiglitazone-induced increase of GPR43 transcription requires dimerization of PPAR and RXR in brown adipocytes. Undifferentiated IM-BAT cells were differentiated in IBMX, dexamethasone, insulin, and T3 containing DMEM:F12 medium for 2 days, followed by insulin and T3 containing medium in the absence or in the presence of rosiglitazone (1 μM) as indicated. The PPARγ antagonist GW9662 (1 μM) (a) or RXR antagonist HX531 (1 μM) (b) were also added to insulin and T3 containing medium during the course of differentiation from day 3 to day 7 after differentiation. mRNA transcription levels of GPR43 in mature adipocytes were measured by quantitative real-time PCR. Data are expressed as mean ± SEM (). by one-way ANOVA followed by Tukey’s multiple comparison.

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