Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury

Figure 2

Neural stem cells from rat spinal cord. (a)-(a1)-(a2) Cells gather into neurospheres. (b1) DAPI immunofluorescence staining. (b2) Nestin-CY3 immunofluorescence staining. (b) Nestin-CY3-DAPI-immunofluorescence staining (merge). Immunofluorescence staining was performed to identify neural stem cells, Nestin /DAPI >90%. Each experiment was repeated three times.