Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells

Figure 4

MEKT1-mediated effect on AtT20 cell proliferation and apoptosis. (a) AtT20 cells were incubated for 96 hours either in the presence of MEKT1 (1 nM, 10 nM, 100 nM, 1 μM, or 10 μM) or DMSO (0.1%) as a control for 24 hours before assay. (b) AtT20 cells were incubated for 96 hours either in the presence of pioglitazone (1 nM, 10 nM, 100 nM, 1 μM, or 10 μM) or DMSO (0.1%) as a control for 24 hours before assay. (c) AtT20 cells were incubated for 96 hours either in the presence of rosiglitazone (1 nM, 10 nM, 100 nM, 1 μM, or 10 μM) or DMSO (0.1%) as a control for 24 hours before assay. Data are expressed as percentages (100%) of control. (d) Effects of MEKT1 on mRNA expression of mPttg dose-dependently. AtT20 cells were treated with MEKT1 (1 nM, 10 nM, 100 nM, 1 μM, or 10 μM) or 0.1% DMSO (vehicle control) for 24 hours. (e) Effects on MEKT1 (10 μM) on AtT20 cell apoptosis. Each point indicates mean ± SEM (). NS stands for “not significant.”

(a)

(b)

(c)

(d)

(e)