Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells

Figure 5

Involvement of PPAR-γ in the MEKT1 effects on Pomc mRNA expression. Effects of PPAR-γ knockdown by its siRNA on (a) PPAR-γ mRNA expression, (b) PPAR-α mRNA expression, and (c) PPAR-β mRNA expression. AtT20 cells transiently transfected with siRNA (negative control; NC or PPAR-γ) for 48 hours were incubated with 0.1% DMSO (control) for 24 hours. Results are expressed as percentages of each control. Each point represents mean ± SEM (). versus basal negative control siRNA. NS stands for “not significant.” (d) Effects of PPAR-γ knockdown by its siRNA on the PPAR-γ protein expression. (e) Effects of PPAR-γ knockdown by its siRNA on the Pomc mRNA expression. AtT20 cells transiently transfected with siRNA (negative control; NC or PPAR-γ) for 48 hours were incubated in the presence of either MEKT1 (10 μM) or 0.1% DMSO (control) for 24 hours, respectively. Data are expressed as percentages (100%) of control. Each point represents mean ± SEM (). NS stands for “not significant.” versus negative control siRNA at 10 μM MEKT1.

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