Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells

Table 1

Primer sequences for RT-qPCR.

Mouse Pomc	Forward	5′-CAGTGCCAGGACCTCACC-3′
	Reverse	5′-CAGCGAGAGGTCGAGTTTG-3′
Mouse PPAR-γ1	Forward	5′-TTCTGACAGGACTGTGTGACAG-3′
	Reverse	5′-ATAAGGTGGAGATGCAGGTTC-3′
Mouse PPAR-α	Forward	5′-AGACACGCAGACGGGTTG-3′
	Reverse	5′-GAGGATGCCACTCCCAGA-3′
Mouse PPAR-β	Forward	5′-TGGAGCTCGATGACAGTGAC-3′
	Reverse	5′- GTACTGGCTGTCAGGGTGGT-3′
Mouse Nur77	Forward	5′-GCACAGCTTGGGTGTTGATG-3′
	Reverse	5′-CAGACGTGACAGGCAGCTG-3′
Mouse Nurr1	Forward	5′-TCAGAGCCCACGTCGATT-3′
	Reverse	5′-TAGTCAGGGTTTGCCTGGAA-3′
Mouse NeuroD1	Forward	5′-ACGCAGAAGGCAAGGTGTCC-3′
	Reverse	5′-TTGGTCATGTTTCCACTTCC-3′
Mouse Tpit	Forward	5′-GCCAGCATGTGACCTACTCTCACT-3
	Reverse	5′-AGTCCAGCTGTCAGGTCCCGAGAA-3′
Mouse Pitx1	Forward	5′-CGGTGTGGACCAACCTCACTGAA-3′
	Reverse	5′-GAGTTGCACGTGTCCCGGTAGA-3′
Mouse NFκB1	Forward	5′-GAAATTCCTGATCCAGACAAAAAC-3′
	Reverse	5′-ATCACTTCAATGGCCTCTGTGTAG-3′
Mouse NFκB2	Forward	5′-CTGGTGGACACATACAGGAAGAC-3′
	Reverse	5′-ATAGGCACTGTCTTCTTTCACCTC-3′
Mouse Pttg	Forward	5′-CTGGGCACTGGTGTCAAG-3′
	Forward	5′-GCTGTTTTGGTTGGAGGGG-3′
Mouse GAPDH	Forward	5′-ACAGTCCATGCCATCACTGCC-3′
	Reverse	5′-GCCTGCTTCACCACCTTCTTG-3′