6-Gingerol Normalizes the Expression of Biomarkers Related to Hypertension via PPARδ in HUVECs, HEK293, and Differentiated 3T3-L1 Cells

Figure 5

Western blot analyses for PPAR and AMPK in HEK293 cells treated with NaCl, 6- gingerol, and GSK0660, or treated by compound C. (a) 6-Gingerol normalized the abnormal expressions of PPARδ and AMPK in HEK293 cells treated with NaCl. In addition, the effects of 6-gingerol were reversed by PPARδ antagonist. (b) The AMPK antagonist, compound C, did not affect the protein level of PPAR in HEK293 cells. The results are expressed as means ± SEM (n=3). Values were statistically analyzed by unpaired t-test. All experiments were repeated three or more times. The statistical significance between the two experimental groups was marked as p < 0.05 and p < 0.01. Meaning of indications: Ctrl means untreated normal control group, Na means NaCl treated group, NaG means NaCl and 6-gingerol treated group, and NaGG means NaCl, 6-gingerol, and GSK0660 treated group.

(a)

(b)