Peroxisome Proliferator-Activated Receptor- Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p
Figure 2
miR-590-5p is required for the suppression of LOX-1 and LOX-1-dependent inflammatory and oxidative injury in HUVECs induced by ox-LDL. (a) The expressions of miR-590-5p were determined in HUVECs stimulated by ox-LDL at a range of doses (0~50 μg/mL). (b–f) The pMIR-CMV-miR590 and control plasmid were, respectively, transfected into HUVECs 48 h before the cells were stimulated by 20 μg/mL ox-LDL; the protein levels of LOX-1, ICAM-1, VCAM-1, and were detected and quantified by Western blot analysis. (g) The expression of miR-590-5p in HUVECs stimulated by ox-LDL with or without Rosiglitazone (50 μmol/mL). (h–l) The effect of antagomiR-590-5p and the negative control (antagomiR-NC) on Rosiglitazone-induced protein levels of LOX-1, ICAM-1, VCAM-1, and in HUVECs stimulated by ox-LDL (n=4, P<0.05; vs Control group; n=4, # P<0.05 vs. ox-LDL-induced group; $ P<0.05 vs. ox-LDL together with Rog treated group).