Adenosine Receptor A1-A2a Heteromers Regulate EAAT2 Expression and Glutamate Uptake via YY1-Induced Repression of PPARγ Transcription

Figure 3

PPARγ regulated the expression of EAAT2 and glutamate release from astrocytes. (a, b) EAAT2 protein and intracellular glutamate levels in astrocytes under OGD in the absence or presence of 1 μM rosiglitazone were detected by western blotting or with a glutamate assay kit, respectively. The data represent the (). vs. the control group and ^{^} vs. the OGD group. (c) Western blot analysis of the expression of PPARγ in astrocytes treated with 30 nM CCPA and 200 nM SCH58261 that were exposed to OGD for 6 h. D. The PPARγ protein level in astrocytes transfected with A2aAR siRNA for 48 h before being subjected to OGD conditions with 30 nM CCPA was evaluated. (e, f) Western blot analysis of the expression of EAAT2 in astrocytes treated with 30 nM CCPA or 200 nM SCH58261 in the presence of 30 μM GW9662, followed by exposure to OGD for 6 h.

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