Adenosine Receptor A1-A2a Heteromers Regulate EAAT2 Expression and Glutamate Uptake via YY1-Induced Repression of PPARγ Transcription

Figure 5

YY1 regulated PPARγ by HDAC1. (a) The PPARγ protein level in astrocytes transfected with YY1 siRNA for 48 h and then exposed to OGD for 6 h was evaluated. (b) The protein level of HDAC1 in astrocytes exposed to OGD for 6 h was measured. (c). Co-IP assays were used to evaluate the interaction between YY1 and HDAC1. (d) The protein level of HDAC1 in astrocytes transfected with HDAC1 siRNA for 48 h was evaluated by western blotting. (e) Luciferase assays evaluated the PPARγ promoter activity in 293 T cells transfected with HDAC siRNA for 48 h and then exposed to OGD for 6 h. The data represent as the (). vs. the NC group and ^{^} vs. the NC/OGD group.

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