Redox Regulation of PPARγ in Polarized Macrophages

Figure 2

Redox status of polarizing MΦ affects proteins located in the nucleus. After cloning of a hybrid protein of the redox marker roGFP2 and the nuclear receptor hPPARγ, this construct was transduced into J774A.1 MΦ (a). J774A.1 MΦ transduced with roGFP2 were used as control. Cellular fractionation (a) was performed to analyze cellular localization of roGFP2 and roGFP2-hPPARγ. J774A.1 MΦ expressing roGFP2-hPPARγ was treated for the indicated times with LPS/IFNγ or IL-4, and the redox status was determined by FACS analysis at 405 nm and 488 nm. Quantification of flow cytometric data is provided in (b). All experiments were performed at least three times. are provided. Untreated control cells were set as 1. (, , ).

(a)

(b)