Ligand-Activated Peroxisome Proliferator-Activated Receptor β/δ Facilitates Cell Proliferation in Human Cholesteatoma Keratinocytes

Figure 3

Expression of PPAR β/δ and ligand activation of target gene (PDK1) in cholesteatoma keratinocytes. (a, c) Expression of PPAR β/δ was quantified by immunoblot after treating with 100 nM GW0742 (a) or 5 μM GSK0660 (c) for 24 h. (e, g) The effect of PPAR β/δ agonist (e) or antagonist (g) on expression of the PPAR β/δ-dependent target gene PDK1 was determined by immunoblot following ligand activation of PPAR β/δ with 100 nM GW0742 or 5 μM GSK0660 for 24 h. (b, d, f, and h) Data are expressed as the of the mean for experiments run in triplicate. (vs. control group).

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