Hydroxychloroquine Potentiates Apoptosis Induced by PPARα Antagonist in 786-O Clear Cell Renal Cell Carcinoma Cells Associated with Inhibiting Autophagy

Figure 1

PPARα was poorly expressed in high differentiation of the ccRCC tissues and 786-O cell line, but highly expressed in poorly differentiation of ccRCC tissues. (a) IHC staining for PPARα among different grades of differentiation in ccRCC tissues when compared with the adjacent normal tissues. (b) Mean OD of IHC staining (normal: adjacent normal tissues; HD: highly differentiation; PD: poorly differentiation). (c) The expression of PPARα mRNA in 786-O and HK-2 cells by qRT-PCR normalized by GAPDH. (d) Western blotting for determining the expression of PPARα protein in 786-O and HK-2 cells. (e) Semiquantification of PPARα in 786-O and HK-2 cells normalized by GAPDH (, unpaired Student’s -test, ).

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